The $100 Million AI Startup Club: 49 Companies Betting on the Future
2025 has matched 2024 as a record year for AI startup funding. 49 US AI startups have raised rounds of $100 million or larger, with approximately $56 billion flowing into the sector. Here's where the money is going and what it tells us about the future of AI.
The Biggest Rounds of 2025
Several funding rounds in 2025 have been historic in scale:
| Company | Amount | Valuation | Focus |
|---|---|---|---|
| OpenAI | $40B | $500B | General AI |
| Anthropic | $13B | $183B | AI Safety |
| Anysphere (Cursor) | $2.3B | $29.3B | AI Coding IDE |
| Thinking Machines Lab | $2B | $12B | AI Research |
| Cerebras Systems | $1.1B | - | AI Chips |
| Groq | $750M | $6.9B | AI Inference |
The Top 5 AI Startups by Funding
1. OpenAI - $40 Billion (March 2025)
OpenAI holds the title for the biggest single round raise of 2025 - and the largest ever by a private tech company. The March round valued the company at approximately $500 billion.
In less than three years, OpenAI has grown from an AI research lab to a $500 billion company spearheading a data center buildout plan endorsed by the White House and partnering with Nvidia.
2. Anthropic - $13 Billion (September 2025)
Anthropic's Series F round in September valued the Claude maker at $183 billion. The round was led by Iconiq, Fidelity, and Lightspeed.
This was Anthropic's second major round of 2025. Earlier in the year, they raised $3.5 billion in a Series E led by Lightspeed with participation from Salesforce Ventures, Menlo Ventures, and General Catalyst, valuing the company at $61.5 billion.
Anthropic has also committed to a $50 billion nationwide AI infrastructure buildout, starting with data centers in Texas and New York.
3. Anysphere (Cursor) - $2.3 Billion (November 2025)
Anysphere, maker of the "vibe coding" platform Cursor, raised $2.3 billion in November at a $29.3 billion valuation. This was the company's second massive round of the year.
Cursor has become the most popular AI-powered IDE, riding the wave of "vibe coding" - the practice of building software by describing it in natural language.
4. Thinking Machines Lab - $2 Billion (July 2025)
This AI research lab confirmed a $2 billion seed round in July, led by Andreessen Horowitz with participation from Nvidia, Accel, and AMD. The round valued the company at $12 billion.
A $2 billion seed round is extraordinary - reflecting investor appetite for early-stage AI research bets.
5. Cerebras Systems - $1.1 Billion
Cerebras pulled in $1.1 billion for its AI chip technology, competing with Nvidia in the AI hardware space.
Notable Mid-Size Rounds
AI Infrastructure
- Lambda: $480 million Series D, valued at ~$2.5 billion (AI cloud computing)
- Groq: $750 million Series D-3, valued at ~$6.9 billion (AI inference hardware)
AI Agents and Applications
- Cognition AI: $400 million Series C, valued at $10.2 billion (creator of Devin, the AI coding agent). Led by Founders Fund
- Sierra: $350 million, valued at $10 billion+ (Bret Taylor's customer service AI platform). Led by Greenoaks Capital
Legal and Enterprise AI
- Harvey: Two $300 million rounds in 2025, jumping from $3 billion to $5 billion valuation in just four months (AI for legal)
Healthcare AI
- Abridge: $550 million across two funding cycles, reaching $5.3 billion valuation (medical documentation AI)
- Hippocratic AI: Two rounds totaling $267 million (healthcare AI)
- OpenEvidence: $210 million at $3.5 billion valuation (AI search tool for clinicians)
Investment Trends
Where Money Is Flowing
Enterprise AI and infrastructure companies form the largest group of companies securing $100M+ rounds. Key themes:
- AI Infrastructure: Chips, cloud computing, inference optimization
- Developer Tools: Cursor, Cognition AI (Devin), coding assistants
- Healthcare: Medical documentation, clinical decision support
- Enterprise Agents: Customer service, sales automation
- Legal Tech: Document analysis, research automation
Speed of Fundraising
According to TechCrunch, several companies founded in 2022-2024 secured nine-figure funding rounds within 1-2 years of launching. This indicates:
- Accelerated development cycles in AI
- Investors' willingness to make large, early bets
- Intense competition for promising AI startups
Multiple Rounds Per Year
Many companies raised multiple large rounds in 2025:
- Anthropic: $3.5B Series E + $13B Series F
- Harvey: Two $300M rounds
- Hippocratic AI: Two rounds totaling $267M
- Abridge: Two rounds totaling $550M
The Investor Landscape
Key investors backing AI startups in 2025:
- Andreessen Horowitz (a16z): Leading rounds in Thinking Machines Lab, others
- Founders Fund: Leading Cognition AI round
- Lightspeed Ventures: Leading Anthropic rounds
- Nvidia: Strategic investor in multiple AI infrastructure companies
- Iconiq: Leading Anthropic Series F
- Greenoaks Capital: Leading Sierra round
What This Tells Us About AI's Future
1. Infrastructure Is Critical
The massive investments in chips (Cerebras, Groq), cloud (Lambda), and inference show that AI's scaling needs aren't going away. Companies need hardware and infrastructure to run increasingly large models.
2. Developer Tools Are Hot
Cursor's $29.3B valuation for an AI coding IDE shows how valuable developer productivity tools are. Cognition AI's $10.2B valuation for an AI coding agent reinforces this.
3. Healthcare Is a Priority
Multiple healthcare AI companies raised hundreds of millions. This sector has clear use cases (documentation, diagnosis assistance) and willing buyers (health systems drowning in paperwork).
4. Enterprise AI Is Maturing
Companies like Sierra (customer service), Harvey (legal), and others are targeting specific enterprise verticals with tailored AI solutions rather than competing directly with OpenAI/Anthropic on general AI.
5. Speed Matters
The compressed timelines from founding to nine-figure raises show that AI moves fast. Companies that can execute quickly are being rewarded with massive capital.
What Isn't Getting Funded
Notable by their absence:
- Consumer AI apps: Most funding goes to enterprise and infrastructure
- AI-only chatbots: Hard to compete with free ChatGPT/Claude/Gemini
- AI without clear use cases: Investors want specific applications, not "AI for AI's sake"
Looking Ahead
With $56 billion flowing into 49 companies just in the US, and matching 2024's record pace, AI funding shows no signs of slowing. The key question: which of these bets will pay off?
History suggests many won't. But the few that do could be worth trillions - which is why investors keep writing nine and ten-figure checks.
Sources
- TechCrunch: Here are the 49 US AI startups that have raised $100M or more in 2025
- Eqvista: Top US AI Funding Rounds - $100M+ in 2025
- Seven Square Tech: Top AI Startups Raising $100M+ in 2025
- Inc: These 5 AI Startups Raised the Most Money in 2025
- CNBC: OpenAI's dominance is unlike anything Silicon Valley has ever seen
- CNBC: Anthropic to spend $50 billion on U.S. AI infrastructure
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